Generator

Part:BBa_K143077

Designed by: Chris Hirst   Group: iGEM08_Imperial_College   (2008-10-27)

P43-gsiB GFP expression construct


GFP gene under expresson of the P43 promoter and gsiB RBS of B. subtilis, with a chloramphenicol resistance marker for ease of selection.


Also contains the 5' and 3' amyE integration sequences to allow integration into B. subtilis at the amyE locus.


This device was used by the Imperial iGEM 2008 team to characterise the P43 promoter and gsiB RBS (Combined part BBa_K143050) as part of the Biofabricator subtilis project.


Sequence and Features



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2141


[edit]
Categories
//chassis/prokaryote/Bsubtilis
Parameters
None